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KMID : 1123920030170051243
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 5 p.1243 ~ p.1250
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Anticancer Effect of Combination with Paljinhangahm-dan and Adriamycin on HepG2 Human Malignant Hepatoma Cell Line
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Baek Eun-Ki
Moon Goo
Won Jin-Hee
Kim Dong-Woung
Baek Dong-Gi
Yoon Jun-Chul
Song Bong-Gil
Lee Byung-Ho
Park Sang-Gu
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Abstract
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This study was designed to elucidate the synergistic cytotoxic mechanisms of the co-treatment of adriamycin and Paljinhangahm-dan in human hepatoma malignant cancer cell line, HepG2. The combination of adriamycin and the ethanol extract of Paljinhangahm-dan synergistically augmented the cytotoxicity of Adriamycin and Paljinhangahm-dan in HepG2 cells. The cytotoxicity of two drugs was revealed as apoptosis characterized by DNA fragmentaton in agarose gel electrophoresis. The apoptotic cytotoxicity of adriamycin and Paljinhangahm-dan was accompanied by the cleavage of procaspase -3 protease and PARP. Of note, anti apoptotic Bcl2 protein was obviously decreased, but Fas was dramatically increased in HepG2 cells co-treated with Adriamycin and Paljinhangahm -dan. In addition, through 2-D gel electorphoresis, we observed that the expression levels of a lot of proteins were obviously changed by the status of drug treatments. This results suggest that the synergistic cytotoxicity of the co-treatment of adriamycin and Paljinhangahm-dan might be caused by the changes of the expression levels of a lot of proteins which play pivotal roles in cell survival or death.
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KEYWORD
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Paljinhangahm-dan(八珍抗癌丹), adriamycin, anticancer effect
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